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Generation of a Universal Human Complement Source by Large-Scale Depletion of IgG and IgM from Pooled Human Plasma
- Source :
- Methods in Molecular Biology ISBN: 9781071618998
- Publication Year :
- 2021
- Publisher :
- Springer US, 2021.
-
Abstract
- Complement is a key component of functional immunological assays used to evaluate vaccine-mediated immunity to a range of bacterial and viral pathogens. However, standardization of these assays is complicated due to the availability of a human complement source that lacks existing antibodies acquired either through vaccination or natural circulation of the pathogen of interest. We have developed a method for depleting both IgG and IgM in 200 mL batches from pooled hirudin-derived human plasma by sequential affinity chromatography using a Protein G Sepharose column followed by POROS™ CaptureSelect™ IgM Affinity resin. The production of large IgG- and IgM-depleted batches of human plasma that retains total hemolytic and alternative pathway activities allows for improved assay standardization and comparison of immune responses in large clinical trials.
Details
- ISBN :
- 978-1-07-161899-8
- ISBNs :
- 9781071618998
- Database :
- OpenAIRE
- Journal :
- Methods in Molecular Biology ISBN: 9781071618998
- Accession number :
- edsair.doi...........93838579e3eb77b55c1ea5e0b3aafabc