A Rapid and Efficient Plant Regeneration Protocol using Leaves and Stems with Buds of Euphorbia pulcherrima Willd. ex Klotzsch (Euphorbiaceae)

Background: Euphorbia pulcherrima is highly ornamental value of poinsettia flower, which posses colorful bracts and low temperature resistant leave. Its embryos or adventitious buds were cultivated mainly from stems, petioles and tender buds. The cultivation of seedlings is studied firstly for improving the quality of Poinsettia. Methods: A rapid and reproducible regeneration system of leaves and stems with buds has been established. There is a comparative analysis of the results of tissue culture of different varieties of Poinsettia. Result: The best medium component of hormones about leaf callus of poinsettia inducing cluster buds formation comprise 6-BA 1.0 mg/L, KT 0.4 mg/L, NAA 0.1 mg/L and 2,4-D 0.05 mg/L. Its medium component inducing cluster buds proliferation include 6-BA 1.2 mg/L and NAA 0.05 mg/L. The medium component about stem with bud inducing cluster buds to germinate comprise 6-BA 1.5 mg/L and NAA 0.1 mg/L. Its medium component inducing cluster buds proliferation include 6-BA 1.0 mg/L and NAA 0.05 mg/L. The different hormones and different addition level of Ac have effect on the length of the root and quantity of the first level root and the second level root.