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Antimitochondrial antibodies of immunoglobulin G3 subclass are associated with a more severe disease course in primary biliary cirrhosis

Authors :
George N. Dalekos
Diego Vergani
George K. Koukoulis
E T Davies
Eirini I. Rigopoulou
Maria G. Mytilinaiou
Dimitrios-Petrou Bogdanos
Christos Liaskos
Source :
Liver International. 27:1226-1231
Publication Year :
2007
Publisher :
Wiley, 2007.

Abstract

Background/Aims: Primary biliary cirrhosis (PBC) is characterised by the presence of immunoglobulin (Ig) G antimitochondrial antibodies (AMA), which are routinely detected by indirect immunofluorescence (IFL) using composite rodent tissue substrate. The IgG subclass distribution and clinical significance of IFL-detected AMA in patients with PBC have not been previously studied in detail. Methods: We have examined IgG subclass-specific AMA detected by IFL on rodent liver, kidney and stomach tissue substrate using affinity-purified IgG subclass monospecific antisera as revealing reagents in 95 AMA-positive PBC patients from Greece. Results: AMA of any of the IgG1, IgG2 or IgG3 subclasses were present in 89/95 (93.7%) patients. Among those 89, 55 (61.8%) had IgG1, 2, 3 AMA positivity; eight (9%) had IgG1, 2; seven (7.9%) had IgG2, 3; eight (9%) had IgG1, 3; nine (10.1%) had IgG1 subclass and two (2.2%) single IgG3 AMA reactivity. IgG4 AMA was absent. IgG3 titres were higher than IgG2 and IgG1 (P

Details

ISSN :
14783223
Volume :
27
Database :
OpenAIRE
Journal :
Liver International
Accession number :
edsair.doi...........94c64bd71794469da0d766fc619bf525
Full Text :
https://doi.org/10.1111/j.1478-3231.2007.01586.x