Viridibacillus Soli sp.nov., Isolated from Forest Soil in Ailaoshan National Nature Reserve

A Gram-staining positive, rod-shaped, and subterminal endospore-forming bacterium, designated strain YIM B01967T, was isolated from a forest soil sample collected in Ailaoshan National Nature Reserve, Yuxi City, Xinpin county, Yunnan province, China. Strain YIM B01967T showed the highest 16S rRNA gene sequence similarity with Viridibacillus arvi (99.05%) and Viridibacillus arenosi (98.92%). Based on the phylogenetic and 16S rRNA gene sequence results, strain YIM B01967T was affiliated to the genus Viridibacillus. The growth of YIM B01967T was observed at 15-35 °C (optimum, 28 °C), pH 7.0-9.0 (optimum, pH 7.5) and in the presence of 0-2% (w/v) NaCl (optimum in 2% NaCl). The cell-wall sugars of YIM B01967T were ribose, glucose, arabinose, galactose, and mannose. The quinone system consisted of the major compound MK-8 and moderate amounts of MK-7. The major fatty acids (> 10%) were iso-C15:0, anteiso-C15:0, C16:1 ω10c. The major polar lipids profile included DPG, PME. The cell wall peptidoglycan was the type A4a with an L-Lys–D-Asp interpeptide bridge. The genomic DNA G+C content of strain YIM B01967T was 36.3 mol%. The ANI value and dDDH values between strain YIM B01967T and Viridibacillus arvi DSM 16317T were 61.03% and 32.1% based on the draft genome sequence. The results support the conclusion that strain YIM B01967T represents a novel species of the genus Viridibacillus, for which proposed the name Viridibacillus soli sp. nov. The type strain is YIM B01967T (= KCTC 43249T = CGMCC 1.18436T).