Generation of Monodisperse Droplets from Tens of $\mu\mathrm{L}$ Sample Volume using Centrifuge-Based Microfluidic Device

We propose a centrifuge-based microfluidic device housed in a standard microtube for generation of monodisperse microdroplets from tens of $\mu \mathrm{l}$ sample volume. Centrifugation drives overpressure of a dispersed phase, thus infuses the dispersed phase into a microchannel. Because of the channel geometry for step emulsification, the infused dispersed phase is pinched off forming droplets. Then, the droplets accumulate on the bottom of the microtube by centrifugal force. We succeeded in generation of monodisperse droplets with a diameter of $130\ \mu\mathrm{m}(\mathrm{CV} by operation of centrifuge. Since the device can produce droplets in a microtube without complex experimental setup (e.g. syringe pump), it will be a powerful tool for simple generation of droplets for droplet-based applications.