Extraction Process Optimization of Fucoidan from Dealginated Kelp Waste

In order to optimize the extraction process of fucoidan from dealginated kelp waste. On the basis of single-factor experiments, Box-Behnken design was evaluated to study different variables of pH, extraction temperature and extraction time on fucoidan yield. By solving the regression equations and analyzing 3-D plots, the optimum conditions were at pH4.9, liquid/material ratio of 20:1 mL/g, extraction temperature of 73°C, and extraction time of 4 h. Under these conditions, the fucoidan yield of dealginated Kelp waste was 1.63 ± 0.07%, which were in good agreement with the predicted values. It is the first report of fucoidan extraction from dealginated kelp waste and the results provides a basis for further purification and application of fucoidan.