The Process Conditions of Extracting Polysaccharides from Dendrocalamus latiflorus by Cellulase

The study is focused on the extracting polysaccharide from Dendrocalamus latiflorus with assisted enzyme and cool water to maintain its biological activity. The optimized conditions for the cellulase process are listed as follows The enzymatic hydrolysis temperature stays 50°C. The mass fraction of cellulase (MFC) is 0.4% on the basis of the mass of dry leaves. The volume of water added in is 20mL on the basis of the mass of dry leaves and the pH value of buffer solution remains 4.6. Under these conditions, the ratio of polysaccharides (RP) can be 0.92% after two extractions for 180min in total and its RP is 12.05% higher than the traditional process.