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Improving sulforaphane content in transgenic broccoli plants by overexpressing MAM1, FMOGS–OX2, and Myrosinase
- Source :
- Plant Cell, Tissue and Organ Culture (PCTOC). 146:461-471
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Sulforaphane (SF) is a potential secondary metabolite with anticancer activity and its metabolic pathway has been revealed in the model plant Arabidopsis thaliana. Generally, the SF extraction cycle from broccoli is long and the yield is low, which means that it no longer meets cancer treatment requirements. Therefore, the SF content needs to be urgently improved. In this study, MAM1, FMOGS–OX2, and Myrosinase, which are required for SF biosynthesis, were introduced into broccoli by Agrobacterium tumefaciens-mediated transformation. To obtain plants with a higher SF content, broccoli was transformed using Myrosinase-FMOGS–OX2-MAM1 (M-F-A) triple genes in tandem and by adding each of the genes on their own. The results showed that the SF contents in the MAM1, FMOGS–OX2, or Myrosinase transgenic plants improved by 1.7–3.4, 1.6–2.7, and 3.7-fold compared to the wild type (WT), respectively, However, the SF contents in transgenic plants with all three genes improved by 1.86–5.5fold. Furthermore, we examined the anticancer effect of the SF extracts from transgenic plants via cell viability detection of HCT116 colon cancer cells. The results showed that SF extracts from transgenic plants had more obvious anticancer activity than SF extracts from WT, and the SF extracts from the multiple genes plants had stronger anticancer activity than the single gene plants. In summary, the multiple genes transformation increased the SF content in broccoli more than single gene introductions. The aim of this research was to improve sulforaphane content by introducing FMOGS–OX2, MAM1, and Myrosinase genes, and Myrosinase-FMOGS–OX2-MAM1 (M-F-A) serial triple genes into broccoli.
- Subjects :
- 0106 biological sciences
biology
Myrosinase
Agrobacterium
Transgene
food and beverages
Genetically modified crops
Horticulture
biology.organism_classification
01 natural sciences
Metabolic pathway
chemistry.chemical_compound
Transformation (genetics)
Biochemistry
chemistry
Arabidopsis thaliana
010606 plant biology & botany
Sulforaphane
Subjects
Details
- ISSN :
- 15735044 and 01676857
- Volume :
- 146
- Database :
- OpenAIRE
- Journal :
- Plant Cell, Tissue and Organ Culture (PCTOC)
- Accession number :
- edsair.doi...........965bbf2feaf07322f06ef009810d0b10