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Detection of surface antibodies against pancreatic B cells in insulin-dependent diabetes
Detection of surface antibodies against pancreatic B cells in insulin-dependent diabetes
- Source :
- Pediatric Research. 22:218-218
- Publication Year :
- 1987
- Publisher :
- Springer Science and Business Media LLC, 1987.
-
Abstract
- Islet cell surface antibodies (ICSA) have been detected in insulin-dependent diabetes (IDD). Current techniques for the detection of ICSA often suffer from a lack of standardization and from a poorly defined specificity. We have developed a standardized method to quantify binding of circulating IgM and IgG fractions with the surface of purified rat pancreatic B cells; parallel experiments on islet non-B cells serve as control for islet cell specificity. Using this procedure no abnormalities were detected in the binding of circulating IgM from 30 recent onset IDD to islet B or non-B cells, comparison being made with 30 healthy age-matched controls. On the other hand, the IgG fraction from IDD patients was found to bind selectively to islet B cells (p 0.01) while no binding was observed with IgG from the normal controls. It is concluded that the developed procedure allows the recognition, quantification and specification of autoimmune reactions against surface components of islet cells.
- Subjects :
- Pancreatic B-cells
endocrine system
medicine.medical_specialty
geography
geography.geographical_feature_category
Cell
Biology
medicine.disease
Islet
medicine.anatomical_structure
Endocrinology
Internal medicine
Diabetes mellitus
Insulin dependent diabetes
Pediatrics, Perinatology and Child Health
medicine
biology.protein
Autoimmune Reactions
Antibody
Recent onset
Subjects
Details
- ISSN :
- 15300447 and 00313998
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Pediatric Research
- Accession number :
- edsair.doi...........9711f9a02d2aee38cc7188a25f2d97d4
- Full Text :
- https://doi.org/10.1203/00006450-198708000-00029