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Detection of surface antibodies against pancreatic B cells in insulin-dependent diabetes

Detection of surface antibodies against pancreatic B cells in insulin-dependent diabetes

Authors :
Frans Gorus
M. Van De Winkel
O. Segers
Daniel Pipeleers
Martine Vercammen
N Balduck
Guido Somers
Source :
Pediatric Research. 22:218-218
Publication Year :
1987
Publisher :
Springer Science and Business Media LLC, 1987.

Abstract

Islet cell surface antibodies (ICSA) have been detected in insulin-dependent diabetes (IDD). Current techniques for the detection of ICSA often suffer from a lack of standardization and from a poorly defined specificity. We have developed a standardized method to quantify binding of circulating IgM and IgG fractions with the surface of purified rat pancreatic B cells; parallel experiments on islet non-B cells serve as control for islet cell specificity. Using this procedure no abnormalities were detected in the binding of circulating IgM from 30 recent onset IDD to islet B or non-B cells, comparison being made with 30 healthy age-matched controls. On the other hand, the IgG fraction from IDD patients was found to bind selectively to islet B cells (p 0.01) while no binding was observed with IgG from the normal controls. It is concluded that the developed procedure allows the recognition, quantification and specification of autoimmune reactions against surface components of islet cells.

Details

ISSN :
15300447 and 00313998
Volume :
22
Database :
OpenAIRE
Journal :
Pediatric Research
Accession number :
edsair.doi...........9711f9a02d2aee38cc7188a25f2d97d4
Full Text :
https://doi.org/10.1203/00006450-198708000-00029