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Hyperosmotic stress rapidly generates lyso-phosphatidic acid in Chlamydomonas
- Source :
- The Plant Journal. 25:541-548
- Publication Year :
- 2001
- Publisher :
- Wiley, 2001.
-
Abstract
- Plant cells are continuously exposed to environmental stresses such as hyper-osmolarity, and have to respond in order to survive. When 32P-labelled Chlamydomonas moewusii cells were challenged with NaCl, the formation of a new radiolabelled phospholipid was stimulated, which was barely detectable before stimulation. The phospholipid was identified as lyso-phosphatidic acid (LPA), and was the only lyso-phospholipid to be accumulated. The increase in LPA was dose- and time-dependent. When other osmotically active compounds were used, the formation of LPA was also induced with similar kinetics, although salts were better inducers than non-salts. At least part of the LPA was generated by phospholipase A2 (PLA2) hydrolysing phosphatidic acid (PA). This claim is based on PA formation preceding LPA production, and PLA2 inhibitors decreasing the accumulation of LPA and promoting the conversion of PA to diacylglycerol pyrophosphate. The latter is another metabolic derivative of PA that is implicated in cell signalling. The involvement of multiple lipid-signalling pathways in hyperosmotic stress responses is discussed.
- Subjects :
- chemistry.chemical_classification
biology
Osmotic shock
Chlamydomonas
Phospholipid
Cell Biology
Plant Science
Phosphatidic acid
biology.organism_classification
chemistry.chemical_compound
Enzyme activator
Enzyme
Phospholipase A2
chemistry
Biochemistry
Lysophosphatidic acid
Genetics
biology.protein
lipids (amino acids, peptides, and proteins)
biological phenomena, cell phenomena, and immunity
Subjects
Details
- ISSN :
- 09607412
- Volume :
- 25
- Database :
- OpenAIRE
- Journal :
- The Plant Journal
- Accession number :
- edsair.doi...........979075e5c21e54b8c3ea5e80922f24b1