Washing Protocol for Intact Proteoform MALDI on Human Pancreas v1

Scope: A detailed protocol entailing the sample washing protocols developed for human pancreas, this includes several timed washing steps for fixing the tissue, removing small molecule interferents, and desalting the tissue surface optimized for use with a Spectroglyph EP-MALDI-2 source mounted on a custom Thermo Scientific UHMR Q Exactive HF Orbitrap. Expected Outcomes: Human pancreas slides ready for pre-extraction and/or matrix application.