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Solution NMR structure of zinc finger 4 and 5 from human INSM1, an essential regulator of neuroendocrine differentiation
- Source :
- Proteins: Structure, Function, and Bioinformatics. 85:957-962
- Publication Year :
- 2017
- Publisher :
- Wiley, 2017.
-
Abstract
- Human INSM1 containing five C-terminal C2H2-type zinc fingers (ZFs), is a key regulator of neuroendocrine development. Previous research reported that full-length INSM1 containing all five ZFs recognized a consensus DNA sequence. Structure elucidation of human INSM1 ZFs is currently insufficient to understand the DNA binding mechanism. Herein, we present the solution NMR structure of ZF4-5, in which the two ZFs adopt a head-to-tail arrangement and each ZF features a canonical ββα fold. NMR titrations and isothermal titration calorimetry experiments showed that ZF4-5 binds weakly to the consensus DNA sequence. Proteins 2017; 85:957-962. © 2016 Wiley Periodicals, Inc.
- Subjects :
- 0301 basic medicine
Zinc finger
Stereochemistry
Regulator
Isothermal titration calorimetry
Biochemistry
Neuroendocrine differentiation
Solution structure
DNA sequencing
03 medical and health sciences
chemistry.chemical_compound
030104 developmental biology
chemistry
Structural Biology
Titration
Molecular Biology
DNA
Subjects
Details
- ISSN :
- 08873585
- Volume :
- 85
- Database :
- OpenAIRE
- Journal :
- Proteins: Structure, Function, and Bioinformatics
- Accession number :
- edsair.doi...........99b2d91deba97a5dd149d09d50fbdf41