Effect of EMP on lipid peroxidation of human gastric cancer cells MKN28

AIM To discuss the effect of EMP on lipid peroxidation of human gastric cancer cells MKN28. METHODS MKN28 cells were divided into 5 groups (control group, 50, 100, 200, 400kVldrm-1groups), 16 wells for each group. 12 hours after the cell-planting, each group were exposed by 0, 50, 100, 200, 400kV-m-1 respectively. 0, 6, 12, 24, 48h after EMP irradiation, the wells of each group were separated and detected by chemiluminescence analysator. The result was recorded in forms of value of first peak and 5 s integral area. The data obtained from the experiment were analyzed by ANOVA. RESULTS Compared with control group, the chemoluminescence intensity (value of first peak and 5 s integral area) of 50, 100kVldrm-1 irradiation groups (0, 6, 24h after irradiation) and 200, 400kVldrm1 irradiation groups (0, 6, 12, 24, 48h after irradiation) decreased significantly (p