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Divergent Metabolic Pathways for Propane and Propionate Utilization by a Soil Isolate

Divergent Metabolic Pathways for Propane and Propionate Utilization by a Soil Isolate

Authors :
Jerome J. Perry
J. R. Vestal
Source :
Journal of Bacteriology. 99:216-221
Publication Year :
1969
Publisher :
American Society for Microbiology, 1969.

Abstract

The metabolism of propane and propionate by a soil isolate (Brevibacteriumsp. strain JOB5) was investigated. The presence of isocitrate lyase in cells grown on isopropanol, acetate, or propane and the absence of this inducible enzyme inn-propanol- and propionate-grown cells suggested that propane is not metabolized via C-terminal oxidation. Methylmalonyl coenzyme A mutase and malate synthase are constitutive in this organism. The incorporation of14CO2into pyruvate accumulated during propionate utilization suggests that propionate is metabolized via the methyl-malonyl-succinate pathway. These results were further substantiated by radiorespirometric studies with propionate-1-14C, -2-14C, and -3-14Cas substrate. Propane -2-14Cwas shown, by unlabeled competitor experiments, to be oxidized to acetone; acetone and isopropanol are oxidized in this organism to acetol. Cleavage of acetol to acetate and CO2would yield the inducer for the isocitrate lyase present in propane-grown cells.

Details

ISSN :
10985530 and 00219193
Volume :
99
Database :
OpenAIRE
Journal :
Journal of Bacteriology
Accession number :
edsair.doi...........9beedeeb027ac962d81aa268590a63ee
Full Text :
https://doi.org/10.1128/jb.99.1.216-221.1969