Back to Search
Start Over
Polyketides from the endophytic fungus Phomopsis sp. sh917 by using the one strain/many compounds strategy
- Source :
- Tetrahedron. 73:3577-3584
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- On the basis of One Strain/Many Compounds (OSMAC) strategy, five new polyketides, named phomopsiketones A-C (1–3), (10S)-10-O-β-D-4′-methoxymannopyranosyldiaporthin (7), and clearanol H (10), and two new natural products, 4 and 5, along with three known ones, 6, 8 and 9, were isolated from an endophytic fungus, Phomopsis sp. sh917, harbored in stems of Isodon eriocalyx var. laxiflora. Their structures were elucidated by spectroscopic methods. The absolute configurations of 1, 3, and 7 were further confirmed by single crystal X-ray diffraction experiment. Compounds 2, 4, 6, 9 and 10 were assigned absolute configurations on the basis of circular dichroism (CD) or optical rotation (OR) data comparison. In addition, antiangiogenic activity of compounds 2, 3, 5, and 6 were evaluated by using human umbilical vascular endothelial cells (HUVECs) in a cell-culture model of angiogenesis. Especially, compound 6 sufficiently inhibited vascular endothelial growth factor (VEGF)-induced angiogenic processes in vitro, including migration, invasion, and tube formation of HUVECs.
- Subjects :
- Tube formation
Circular dichroism
Strain (chemistry)
010405 organic chemistry
Chemistry
Stereochemistry
Angiogenesis
Organic Chemistry
Isodon eriocalyx
Endophytic fungus
01 natural sciences
Biochemistry
0104 chemical sciences
Vascular endothelial growth factor
010404 medicinal & biomolecular chemistry
chemistry.chemical_compound
Drug Discovery
Phomopsis sp
Subjects
Details
- ISSN :
- 00404020
- Volume :
- 73
- Database :
- OpenAIRE
- Journal :
- Tetrahedron
- Accession number :
- edsair.doi...........9d5ad973bad98aefdf23446b40d99c56