Regeneration of cotyledonary nodes from the recalcitrant melon cultivar Gacho

There is lack of suitable explants for efficient shoot proliferation in recalcitrant melon cultivars restricts genetic manipulation for crop improvement. In this study, we aimed to stimulate the regeneration capability of cells of melon (Cucumis melo L.) explants in order to increase shoot proliferation efficiency during regeneration. The media of seeds germination containing different concentrations of benzylaminopurine (BAP: 0.5, 1.0, and 1.5 mg L-1) can stimulate the shoot proliferation potential of cotyledonary nodes was described. The highest number of shoots per explant was obtained when the seeds and cotyledonary nodes were cultured on Murashige and Skoog media containing 1.0 and 0.5 mg L-1 BAP, respectively, and the regeneration process occurred through organogenesis. Key words: Cucumis melo L., organogenesis, cytokinin, explants.