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Active N6-Methyladenine Demethylation by DMAD Regulates Gene Expression by Coordinating with Polycomb Protein in Neurons
- Source :
- Molecular Cell. 71:848-857.e6
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- A ten-eleven translocation (TET) ortholog exists as a DNA N6-methyladenine (6mA) demethylase (DMAD) in Drosophila. However, the molecular roles of 6mA and DMAD remain unexplored. Through genome-wide 6mA and transcriptome profiling in Drosophila brains and neuronal cells, we found that 6mA may epigenetically regulate a group of genes involved in neurodevelopment and neuronal functions. Mechanistically, DMAD interacts with the Trithorax-related complex protein Wds to maintain active transcription by dynamically demethylating intragenic 6mA. Accumulation of 6mA by depleting DMAD coordinates with Polycomb proteins and contributes to transcriptional repression of these genes. Our findings suggest that active 6mA demethylation by DMAD plays essential roles in fly CNS by orchestrating through added epigenetic mechanisms.
- Subjects :
- 0301 basic medicine
biology
fungi
Cell Biology
Cell biology
Gene expression profiling
03 medical and health sciences
030104 developmental biology
0302 clinical medicine
Gene expression
DNA methylation
biology.protein
Polycomb-group proteins
Demethylase
Epigenetics
Molecular Biology
Gene
030217 neurology & neurosurgery
Drosophila Protein
Subjects
Details
- ISSN :
- 10972765
- Volume :
- 71
- Database :
- OpenAIRE
- Journal :
- Molecular Cell
- Accession number :
- edsair.doi...........a09219e7b8402b8dc1e24dcd9bea166f