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Optimal Fermentation of Saccharomyces cerevisiae Expressing a Dextranase from Chaetomium gracile
- Source :
- Sugar Tech. 22:171-178
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- Dextranase from Chaetomium gracile is generally considered safe for use in the sugarcane industry. Herein, a truncated and codon-optimised α-dextranase gene from C. gracile was successfully cloned and expressed in Saccharomyces cerevisiae for the first time. The optimum conditions of fermentation was achieved when the maximum dextranase activity reached to 58.45 U/mL after 48 h in shake flasks. The optimal pH and temperature were 5.5 and 60 °C, respectively. The recombinant dextranase remained stable between pH 4 and 6 and temperature between 55 and 60 °C. The findings in the present study could facilitate large-scale production of food-grade recombinant dextranase for use in the sugar industry.
- Subjects :
- 0106 biological sciences
Shake flask
Dextranase
biology
Chemistry
Sugar industry
Saccharomyces cerevisiae
04 agricultural and veterinary sciences
Chaetomium gracile
biology.organism_classification
01 natural sciences
Dextranase activity
law.invention
law
040103 agronomy & agriculture
Recombinant DNA
0401 agriculture, forestry, and fisheries
Fermentation
Food science
Agronomy and Crop Science
010606 plant biology & botany
Subjects
Details
- ISSN :
- 09740740 and 09721525
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Sugar Tech
- Accession number :
- edsair.doi...........a0b95b51a494bb6a8515392248d81762