Synthesis of some Mannich bases of isatin-3-(4′-phenyl-3′-thiosemicarbazone) and their antibacterial activity

Some Mannich bases of isatin-3-(4′-phenyl-3′-thiosemicarbazone) have been prepared by employing formaldehyde and different secondary amines. These Mannich bases have been characterized on the basis of different physico-chemical evidences. These behave as Lewis-bases and have been estimated accordingly in non-aqueous media. Like some alkaloids they also form the reineckate complexes which serve for their estimation. Antibacterial activity of the synthesized Mannich bases has been studied by employingEscherichia coli andStaphylococcus aureus as bacterial strains.