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Feasibility of clinical detection of cervical dysplasia using angle-resolved low coherence interferometry measurements of depth-resolved nuclear morphology
- Source :
- International Journal of Cancer. 140:1447-1456
- Publication Year :
- 2017
- Publisher :
- Wiley, 2017.
-
Abstract
- This study sought to establish the feasibility of using in situ depth-resolved nuclear morphology measurements for detection of cervical dysplasia. Forty enrolled patients received routine cervical colposcopy with angle-resolved low coherence interferometry (a/LCI) measurements of nuclear morphology. a/LCI scans from 63 tissue sites were compared to histopathological analysis of co-registered biopsy specimens which were classified as benign, low-grade squamous intraepithelial lesion (LSIL), or high-grade squamous intraepithelial lesion (HSIL). Results were dichotomized as dysplastic (LSIL/HSIL) versus non-dysplastic and HSIL versus LSIL/benign to determine both accuracy and potential clinical utility of a/LCI nuclear morphology measurements. Analysis of a/LCI data was conducted using both traditional Mie theory based processing and a new hybrid algorithm that provides improved processing speed to ascertain the feasibility of real-time measurements. Analysis of depth-resolved nuclear morphology data revealed a/LCI was able to detect a significant increase in the nuclear diameter at the depth bin containing the basal layer of the epithelium for dysplastic versus non-dysplastic and HSIL versus LSIL/Benign biopsy sites (both p 0.80) in identifying dysplastic biopsies and HSIL. The hybrid algorithm demonstrated a threefold decrease in processing time at a slight cost in classification accuracy. The results demonstrate the feasibility of using a/LCI as an adjunctive clinical tool for detecting cervical dysplasia and guiding the identification of optimal biopsy sites. The faster speed from the hybrid algorithm offers a promising approach for real-time clinical analysis.
- Subjects :
- Colposcopy
Cervical cancer
Cancer Research
Pathology
medicine.medical_specialty
medicine.diagnostic_test
business.industry
Optical Biopsy
medicine.disease
01 natural sciences
010309 optics
Nuclear morphology
03 medical and health sciences
Squamous intraepithelial lesion
0302 clinical medicine
Oncology
Dysplasia
030220 oncology & carcinogenesis
0103 physical sciences
Biopsy
medicine
business
Nuclear medicine
Angle-resolved low-coherence interferometry
Subjects
Details
- ISSN :
- 00207136
- Volume :
- 140
- Database :
- OpenAIRE
- Journal :
- International Journal of Cancer
- Accession number :
- edsair.doi...........a11fceff55250ddba82e605404e4ccf4