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1,25(OH)2D3 Activates Autophagy to Protect against Oxidative Damage of INS-1 Pancreatic Beta Cells
- Source :
- Biological and Pharmaceutical Bulletin. 42:561-567
- Publication Year :
- 2019
- Publisher :
- Pharmaceutical Society of Japan, 2019.
-
Abstract
- Diabetes mellitus is a serious disease endangering human health worldwide. Vitamin D (Vit D) is a well-characterized regulator of calcium-phosphorus metabolism that also exerts other biological effects extending far beyond mineral homeostasis. Some epidemiological studies have suggested that Vit D has a role in defense against diabetes, although the mechanism remains unclear. Autophagy, an intracellular catabolic process, is necessary to maintain the normal structure and function of host cells. In our previous study, we found that Vit D could induce autophagy of pancreatic beta cells and prevent insulitis, although the underlying mechanisms remain to be fully elucidated. In this study, the protective effect of 1,25(OH)2D3, the physiologically active metabolite of Vit D, against streptozotocin-induced cytotoxicity in rat insulinoma cell line (INS-1) cells was explored. Cell viability and insulin secretion of INS-1 cells in response to different treatments were measured with a cell counting kit and enzyme-linked immune absorbent assay (ELISA), respectively. In addition, malondialdehyde (MDA) content and total antioxidant capacity (T-AOC) were measured by ELISA. RT-PCR and Western blot analyses were used to detect autophagy levels, reactive oxygen species (ROS) was assessed by fluorescence microscope, ultrastructure analysis was performed using transmission electron microscopy. The results demonstrated that 1,25(OH)2D3 could increase cell viability and insulin secretion of INS-1 cells, and protected cells from oxidative damage induced by streptozotocin (STZ) through autophagy activation. These findings shed light on mechanisms underlying the ameliorative effects of Vit D on diabetes mellitus.
- Subjects :
- 0301 basic medicine
Pharmacology
chemistry.chemical_classification
Reactive oxygen species
Autophagy
Pharmaceutical Science
General Medicine
medicine.disease
Streptozotocin
03 medical and health sciences
030104 developmental biology
0302 clinical medicine
Immune system
chemistry
Cell culture
030220 oncology & carcinogenesis
medicine
Viability assay
Insulitis
Intracellular
medicine.drug
Subjects
Details
- ISSN :
- 13475215 and 09186158
- Volume :
- 42
- Database :
- OpenAIRE
- Journal :
- Biological and Pharmaceutical Bulletin
- Accession number :
- edsair.doi...........a12df961e9cbdd86bd75bc0c3b4c412c