Degradation of mitomycin C in acidic solution

The degradation of mitomycin C in acidic medium has been investigated utilizing a stability indicating high-performance liquid Chromatographic assay and ultraviolet spectrophotometry. Rate constants for the decomposition at 20°C are determined and a pH profile was constructed. The ratio between the two mitosene degradation products as function of pH is quantified. The degradation mechanism of mitomycin C in acidic solution is evaluated.