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Utilization of Cyclodextrins and Its Derivative Particles as Nucleants for Protein Crystallization
- Source :
- Crystal Growth & Design. 17:6189-6200
- Publication Year :
- 2017
- Publisher :
- American Chemical Society (ACS), 2017.
-
Abstract
- Finding new nucleants to promote protein crystallization is an important task, especially for purposes other than structural determination. Here, we investigated cyclodextrins and its derivative particles, as potential nucleants for protein crystallization. β-cyclodextrin (β-CD) and its derivatives (including p-toluenesulfonyl-β-cyclodextrin (PTCD), polymer-β-cyclodextrin (PCD), Mono-(6-(1,6-hexamethylenediamine)-6-deoxy)-β-Cyclodextrin (MHCD) and Mercapto-β-cyclodextrin (MCD)) were used as nucleants. The experimental results confirmed that β-CD and its derivatives showed significantly positive effects, promoting protein crystallization and improving crystal quality. A larger number of protein molecules (including lysozyme, catalase, subtilisin A VIII, concanavalin A VI, α-chymotrypsinogen, proteinase K, cellulase, papain, glucose isomerase, hemoglobin and ribonuclease A XII) attached to the particles usually corresponded to a higher crystallization success rate. More detailed analysis showed that cyclode...
- Subjects :
- Glucose-6-phosphate isomerase
biology
02 engineering and technology
General Chemistry
Cellulase
010402 general chemistry
021001 nanoscience & nanotechnology
Condensed Matter Physics
Proteinase K
01 natural sciences
Combinatorial chemistry
0104 chemical sciences
law.invention
chemistry.chemical_compound
Papain
chemistry
law
Concanavalin A
biology.protein
Organic chemistry
General Materials Science
Lysozyme
Crystallization
0210 nano-technology
Protein crystallization
Subjects
Details
- ISSN :
- 15287505 and 15287483
- Volume :
- 17
- Database :
- OpenAIRE
- Journal :
- Crystal Growth & Design
- Accession number :
- edsair.doi...........a3376cd57b6718207f18208e92c0ac2b