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Improved multiple rapid immunofilter paper assay to detect three viruses of cucumber simultaneously
- Source :
- Journal of General Plant Pathology. 77:307-311
- Publication Year :
- 2011
- Publisher :
- Springer Science and Business Media LLC, 2011.
-
Abstract
- We improved a multiple rapid immunofilter paper assay (multi-RIPA) to simultaneously detect three major viruses of cucumber, Cucumber mosaic virus, Melon yellow spot virus and Zucchini yellow mosaic virus. When we increased the concentration of bovine serum albumin (BSA) in the extraction buffer, a positive band for each virus developed in the multi-RIPA, regardless of the order that the three sensitized white latex particles were immobilized on the strip. The increase in BSA concentration also strengthened the sensitivity of each RIPA. In addition, using a mixture of commercially available, red and blue, sensitized latex solutions as a tracer, purple bands could be produced on the strips. Because each virus yielded an unique color for a positive reaction, the three viruses were easy to distinguish.
Details
- ISSN :
- 1610739X and 13452630
- Volume :
- 77
- Database :
- OpenAIRE
- Journal :
- Journal of General Plant Pathology
- Accession number :
- edsair.doi...........a3e6af6fb7ee4302034dc3cc6e2a66c4
- Full Text :
- https://doi.org/10.1007/s10327-011-0334-x