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The transmembrane domain of N-glucosaminyltransferase I contains a Golgi retention signal

Authors :
Wanjin Hong
Siew Heng Wong
Bor Luen Tang
Seng Hui Low
Source :
Journal of Biological Chemistry. 267:10122-10126
Publication Year :
1992
Publisher :
Elsevier BV, 1992.

Abstract

The enzyme N-acetylglucosaminyltransferase I (NT, EC 2.4.1.101) is a resident type II transmembrane protein of the Golgi apparatus. To delineate the portion of its primary sequence that is responsible for the Golgi retention of this protein, we constructed chimeras containing different N-terminal portions of NT joined to a reporter sequence, the ectodomain of a type II surface membrane protein. These chimeric proteins were found to be retained in the Golgi apparatus as assessed by cell surface biotinylation and immunofluorescence. We found that the transmembrane domain of NT is sufficient to confer Golgi retention of the fusion proteins and propose that it contains the Golgi retention signal of the parent molecule.

Details

ISSN :
00219258
Volume :
267
Database :
OpenAIRE
Journal :
Journal of Biological Chemistry
Accession number :
edsair.doi...........a50f6172b5717810eff7e9e7e8f5ce86