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Localization of a phosphatidylglycerol/ phosphatidylinositol transfer protein in Aspergillus oryzae
- Source :
- Canadian Journal of Microbiology. 44:945-953
- Publication Year :
- 1998
- Publisher :
- Canadian Science Publishing, 1998.
-
Abstract
- The subcellular localization of the phosphatidylglycerol/phosphatidylinositol transfer protein (PG/PI-TP) of Aspergillus oryzae was investigated using Western blot analysis of the cell protein extracts, a cellular membrane fractionation technique, and transmission electron microscopy. The PG/PI-TP, as detected by Western blot analysis with a specific immune serum, was found to be mainly cytoplasmic and partly associated with intracellular membranes. A fractionation experiment was conducted after homogenization of the filamentous fungus mycelium. The endoplasmic reticulum, Golgi-like vesicles, and the plasma membrane were separated by isopycnic ultracentrifugation on a sucrose gradient, and our data revealed that the immunodetected PG/PI-TP was only associated with the Golgi-like apparatus. All these results were documented by electron microscopy and indicate here for the first time that there exists a specific phospholipid transfer protein in a filamentous fungus that is localized in the cytoplasm and associated with Golgi-like vesicles.Key words: phospholipid transfer protein, subcellular fractionation, ultrastructural localization, filamentous fungus, Aspergillus oryzae.
- Subjects :
- Phosphatidylglycerol
biology
Endoplasmic reticulum
Immunology
General Medicine
Subcellular localization
biology.organism_classification
Applied Microbiology and Biotechnology
Microbiology
Blot
chemistry.chemical_compound
Biochemistry
chemistry
Aspergillus oryzae
Phospholipid transfer protein
Genetics
Phosphatidylinositol
Molecular Biology
Phosphatidylinositol transfer protein
Subjects
Details
- ISSN :
- 14803275 and 00084166
- Volume :
- 44
- Database :
- OpenAIRE
- Journal :
- Canadian Journal of Microbiology
- Accession number :
- edsair.doi...........a5e4a4f942bfaf07a40787476cb6571e
- Full Text :
- https://doi.org/10.1139/w98-092