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Quantification of Protein Concentration Using UV Absorbance and Coomassie Dyes

Authors :
James E. Noble
Publication Year :
2014
Publisher :
Elsevier, 2014.

Abstract

The measurement of a solubilized protein concentration in solution is an important assay in biochemistry research and development labs for applications ranging from enzymatic studies to providing data for biopharmaceutical lot release. Spectrophotometric protein quantification assays are methods that use UV and visible spectroscopy to rapidly determine the concentration of protein, relative to a standard, or using an assigned extinction coefficient. Where multiple samples need measurement, and/or the sample volume and concentration is limited, preparations of the Coomassie dye commonly known as the Bradford assay can be used.

Details

Database :
OpenAIRE
Accession number :
edsair.doi...........a6f0c69f177b677f4f7b80c08b0aa8d2
Full Text :
https://doi.org/10.1016/b978-0-12-420070-8.00002-7