Anthocyanin production in suspension cultures of high-producing cells of Euphorbia millii

We investigated the optimal conditions for anthocyanin production in suspension cultures of a Euphorbia millii cell strain obtained by successive cell-aggregate selections. A modified Gamborg’s solution containing 5 to 7% sucrose (w/v), a 1:16 ratio of , 30 mm of nitrogen source, 10 6 m2,4-D, 10 8 m BA, and low concentrations of Fe2+ and greatly increased anthocyanin production. 2,4-D was superior to NAA in increasing anthocyanin production. In the anthocyanin production medium we established, E. millii cells produced 32 mg anthocyanin per liter per day.