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Continuous production of glycosides by a bioreactor using ginseng hairy root culture
- Source :
- Applied Microbiology and Biotechnology. 39:460-464
- Publication Year :
- 1993
- Publisher :
- Springer Science and Business Media LLC, 1993.
-
Abstract
- Ginseng (Panax ginseng) hairy-root culture, established by transformation with the Ri plasmid of Agrobacterium rhizogenes, had a higher potential to biotransform (RS)-2-phenylpropionic acid (PPA) to (RS)-2-phenylpropionyl β-d-glucopyranoside (1) (71% conversion ratio), (2RS)-2-O-(2-phenylpropionyl)-d-glucose (2) (8%), (2S)-2-phenylpropionyl 6-O-β-d-xylopyranosyl-β-d-glucopyranoside (3) (10%) and a myo-inositol ester of (R)-2-phenylpropionic acid (4) (5%). Moreover, the hairy root excreted about a half of the conversion products, 46.8%. The continuous glycosylation of PPA was carried out using a bioreactor with ginseng hairy root, and the continuous long-term reaction for 2 months was successfully made at a high conversion ratio, 30% or more on average.
Details
- ISSN :
- 14320614 and 01757598
- Volume :
- 39
- Database :
- OpenAIRE
- Journal :
- Applied Microbiology and Biotechnology
- Accession number :
- edsair.doi...........a8c5913c4f45fd64d8599d6e6810688e