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Zebrafishjam-b2Gal4-enhancer trap line recapitulates endogenousjam-b2expression in extraocular muscles
- Source :
- Developmental Dynamics. 244:1574-1580
- Publication Year :
- 2015
- Publisher :
- Wiley, 2015.
-
Abstract
- BACKGROUND Members of the junctional adhesion molecule (JAM) family function as cell adhesion molecules and cell surface receptors. The zebrafish genome contains six different jam genes, and jam-b and jam-c were shown to be essential for myoblast fusion during skeletal muscle development. However, little is known about jam-b2 expression and function. RESULTS We isolated the cDNA of zebrafish jam-b2. jam-b2 is expressed specifically in extraocular muscles (EOMs), jaw muscles, and pectoral fins in zebrafish larvae, but not in trunk muscles. The identified jam-b2 expression pattern is supported by the analysis of a zebrafish Gal4-enhancer trap line, in which the coding sequence of the transcriptional activator KalTA4 together with a Gal4-dependent UAS-mCherry expression cassette was inserted into the jam-b2 locus. Intercrosses with an UAS:EGFP strain proves the possibility for targeting transgene expression to EOMs, jaw muscles and fins. Finally, we characterized the concerted contraction pattern of EOMs in larvae performing an optokinetic response. CONCLUSIONS The expression pattern of jam-b2 suggests that it may contribute different properties to EOMs, jaw muscles, and pectoral fins. The jam-b2:KalTA4-UAS-mCherry transgenic strain serves a dual role as both a reporter for these muscles and as a valuable genetic tool for targeting transgene expression to EOMs.
- Subjects :
- animal structures
Cell adhesion molecule
Transgene
education
fungi
Skeletal muscle
Anatomy
Biology
Extraocular muscles
biology.organism_classification
humanities
Cell biology
Myoblast fusion
medicine.anatomical_structure
cardiovascular system
medicine
Enhancer trap
Expression cassette
Zebrafish
Developmental Biology
Subjects
Details
- ISSN :
- 10588388
- Volume :
- 244
- Database :
- OpenAIRE
- Journal :
- Developmental Dynamics
- Accession number :
- edsair.doi...........a94f7bdee986fcad18354ca686a6d6a5