Back to Search
Start Over
Antiviral Activity of the Sesquiterpene Lactones from Centipeda minima against Influenza a Virus in vitro
- Source :
- Natural Product Communications. 13:1934578X1801300
- Publication Year :
- 2018
- Publisher :
- SAGE Publications, 2018.
-
Abstract
- During the course of searching for antiviral agents from Chinese medicinal herbs, we found that the supercritical fluid extract (SFE) of Centipeda minima possessed good in vitro antiviral activity against influenza virus A/Puerto Rico/8/34 H1N1 (PR8). Bioassay-guided isolation and identification led to the isolation from this extract of seven pseudoguaianolides (1-7). These, as well as nine other sesquiterpene lactones (8-16) previously isolated from this herb were all tested for their anti-PR8 activity using both the cytopathogenic effect (CPE) reduction and cell counting kit 8 (CCK8) assays. As a result, eight pseudoguaianolides (1-8) possessing an α,β-unsaturated cyclopentenone moiety showed antiviral activity against PR8 to different extents. Of the active compounds, brevilin A (4) exhibited the strongest anti-PR8 activity, with an IC50 value much lower than that of the positive control ribavirin. Mechanistic study revealed that brevilin A affected the intracellular replication of PR8 via downregulating the expression of viral M2 protein. All these results suggest the potential application of the pseudoguaianolides containing an α,β-unsaturated cyclopentenone moiety (e.g. brevilin A) in the treatment of influenza virus infection.
- Subjects :
- 0301 basic medicine
Pharmacology
Traditional medicine
viruses
virus diseases
Plant Science
General Medicine
medicine.disease_cause
Sesquiterpene
complex mixtures
01 natural sciences
In vitro
0104 chemical sciences
Centipeda minima
010404 medicinal & biomolecular chemistry
03 medical and health sciences
chemistry.chemical_compound
030104 developmental biology
Complementary and alternative medicine
chemistry
Drug Discovery
Influenza A virus
medicine
Medicinal herbs
Subjects
Details
- ISSN :
- 15559475 and 1934578X
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Natural Product Communications
- Accession number :
- edsair.doi...........a9762aa46b8ae19d734ebe97e95fe0ed