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Valproic Acid Enhances iPSC Induction From Human Bone Marrow-Derived Cells Through the Suppression of Reprogramming-Induced Senescence
- Source :
- Journal of Cellular Physiology. 231:1719-1727
- Publication Year :
- 2015
- Publisher :
- Wiley, 2015.
-
Abstract
- Reprogramming of human somatic cells into pluripotent cells (iPSCs) by defined transcription factors is an extremely inefficient process. Treatment with the histone deacetylase inhibitor valproic acid (VPA) during reprogramming can improve the induction of iPSCs. To examine the specific mechanism underlying the role of VPA in reprogramming, we transfected human bone marrow-derived cells (HSC-J2 and HSC-L1) with lentiviruses carrying defined factors (OCT4, SOX2, KLF4, and c-MYC, OSKM) in the presence of VPA. We found that, OSKM lentiviruses caused significant senescence in transfected cells. Administration of VPA, however, significantly suppressed this reprogramming-induced stress. Notably, VPA treatment improved cell proliferation in the early stages of reprogramming, and this was related to the down-regulation of the activated p16/p21 pathway. In addition, VPA also released the G2/M phase blockade in lentivirus-transfected cells. This study demonstrates a new mechanistic role of the histone deacetylase inhibitor in enhancing the induction of pluripotency. J. Cell. Physiol. 231: 1719-1727, 2016. © 2015 Wiley Periodicals, Inc.
- Subjects :
- 0301 basic medicine
Physiology
medicine.drug_class
Cell growth
fungi
Clinical Biochemistry
Histone deacetylase inhibitor
Cell Biology
Biology
Cell biology
03 medical and health sciences
030104 developmental biology
Biochemistry
SOX2
KLF4
Cell culture
medicine
lipids (amino acids, peptides, and proteins)
Induced pluripotent stem cell
Reprogramming
Cell aging
Subjects
Details
- ISSN :
- 00219541
- Volume :
- 231
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular Physiology
- Accession number :
- edsair.doi...........aaf3514afe2882b0fe6a9bb4f2838deb
- Full Text :
- https://doi.org/10.1002/jcp.25270