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Valproic Acid Enhances iPSC Induction From Human Bone Marrow-Derived Cells Through the Suppression of Reprogramming-Induced Senescence

Authors :
Dehai Yu
Ji-Fan Hu
Xi Chen
Yingying Zhai
Jiuwei Cui
Wei Li
Source :
Journal of Cellular Physiology. 231:1719-1727
Publication Year :
2015
Publisher :
Wiley, 2015.

Abstract

Reprogramming of human somatic cells into pluripotent cells (iPSCs) by defined transcription factors is an extremely inefficient process. Treatment with the histone deacetylase inhibitor valproic acid (VPA) during reprogramming can improve the induction of iPSCs. To examine the specific mechanism underlying the role of VPA in reprogramming, we transfected human bone marrow-derived cells (HSC-J2 and HSC-L1) with lentiviruses carrying defined factors (OCT4, SOX2, KLF4, and c-MYC, OSKM) in the presence of VPA. We found that, OSKM lentiviruses caused significant senescence in transfected cells. Administration of VPA, however, significantly suppressed this reprogramming-induced stress. Notably, VPA treatment improved cell proliferation in the early stages of reprogramming, and this was related to the down-regulation of the activated p16/p21 pathway. In addition, VPA also released the G2/M phase blockade in lentivirus-transfected cells. This study demonstrates a new mechanistic role of the histone deacetylase inhibitor in enhancing the induction of pluripotency. J. Cell. Physiol. 231: 1719-1727, 2016. © 2015 Wiley Periodicals, Inc.

Details

ISSN :
00219541
Volume :
231
Database :
OpenAIRE
Journal :
Journal of Cellular Physiology
Accession number :
edsair.doi...........aaf3514afe2882b0fe6a9bb4f2838deb
Full Text :
https://doi.org/10.1002/jcp.25270