Back to Search
Start Over
In Silico Analysis of Micro-RNA Sequencing Data
- Source :
- Methods in Molecular Biology ISBN: 9781071613061
- Publication Year :
- 2021
- Publisher :
- Springer US, 2021.
-
Abstract
- High-throughput sequencing for micro-RNAs (miRNAs) to obtain expression estimates is a central method of molecular biology. Surprisingly, there are a number of different approaches to converting sequencing output into micro-RNA counts. Each has their own strengths and biases that impact on the final data that can be obtained from a sequencing run. This chapter serves to make the reader aware of the trade-offs one must consider in analyzing small RNA sequencing data. It then compares two methods, miRge2.0 and the sRNAbench and the steps utilized to output data from their tools.
Details
- ISBN :
- 978-1-07-161306-1
- ISBNs :
- 9781071613061
- Database :
- OpenAIRE
- Journal :
- Methods in Molecular Biology ISBN: 9781071613061
- Accession number :
- edsair.doi...........ab79411e0e3c2231b581bac4e8c00de3
- Full Text :
- https://doi.org/10.1007/978-1-0716-1307-8_13