O7 Proteome, biochemical and plasma glucose analysis of Coscinium fenestratum stem extract as an anti-diabetic agent

Coscinium fenestratum is a woody climber found in south East Asia and has been widely used as a medicinal plant. The infusion and tincture preparation of stem is widely used in traditional medicine. Our research aims to investigate the effect of Coscinium fenestratum ( C. fenestratum ) stem extract on plasma glucose, biochemical parameters, antioxidant enzymes and proteome in streptozotocin (STZ) induced diabetic rats. Crude extract from C. fenestratum stem was extracted using Dichloromethane (DCM) and fractionated into fractions using column chromatography. The glucose lowering effect was examined using STZ-induced rats and biochemical parameters and antioxidant enzymes assessed using commercial laboratory services. Proteome analysis was done using two dimensional gel electrophoresis (2-DE) and mass spectrometry (Proteomics technique). Protein–protein interaction network (PPI) was determined by multidimensional protein identification technology (MudPIT) and the interaction between the proteins were analyzed using STRING v10 database. Our findings showed that C. fenestratum stem extract was able to reduce the plasma glucose level, maintain the kidney and liver biomarkers and increase the antioxidant enzymes. Proteome analysis showed elevation of lipid metabolizing proteins, channel proteins, protection proteins, antioxidant proteins, and digestive proteins in the treated group. HPLC analyses of purified fraction E from the stem extract of C. fenestratum have shown that Palmatine is one of the major bioactive components. C. fenestratum stem extract is able to reduce the plasma glucose level, reduce lipids, increase antioxidant enzymes and stimulated the very important lipid metabolizing proteins, channel proteins, protection proteins, antioxidant proteins, and digestive proteins.