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Functional Expression of Thermo-transient Receptor Potential Channels in Dental Primary Afferent Neurons
- Source :
- Journal of Biological Chemistry. 281:17304-17311
- Publication Year :
- 2006
- Publisher :
- Elsevier BV, 2006.
-
Abstract
- Temperature signaling can be initiated by members of transient receptor potential family (thermo-TRP) channels. Hot and cold substances applied to teeth usually elicit pain sensation. This study investigated the expression of thermo-TRP channels in dental primary afferent neurons of the rat identified by retrograde labeling with a fluorescent dye in maxillary molars. Single cell reverse transcription-PCR and immunohistochemistry revealed expression of TRPV1, TRPM8, and TRPA1 in subsets of such neurons. Capsaicin (a TRPV1 agonist), menthol (a TRPM8 agonist), and icilin (a TRPM8 and TRPA1 agonist) increased intracellular calcium and evoked cationic currents in subsets of neurons, as did the appropriate temperature changes (>43 degrees
Details
- ISSN :
- 00219258
- Volume :
- 281
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi...........ad8448834406728f560ad4d1c14532e0