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Additional file 7: of Expression of ID4 protein in breast cancer cells induces reprogramming of tumour-associated macrophages
- Publication Year :
- 2018
- Publisher :
- figshare, 2018.
-
Abstract
- Figure S4 a Modulation of selected genes modulated in the TLDA was validated by RT-qPCR in differentiated HL60 cells cultured in CM from ID4-overexpressing (CM ID4-HA) or control (CM EV) MDA-MB-468 cells (left panel). The same transcripts were analysed in MDA-MB-468 cells transfected with ID4-HA expression vector (ID4-HA) or control empty vector (EV) (right panel). b Expression of EphB2, MDK and GRN protein evaluated by Western blotting on lysates from differentiated HL60 cells cultured as in (a); secreted GRN (sGRN) was evaluated on CM from differentiated HL60 cells in the same conditions. c HIF1A protein expression evaluated by Western blotting in differentiated U937 cells cultured in RPMI medium or in CM from SKBR3 cells stably interfered for ID4 expression (sh-ID4) or control cells (sh-CTR). (PDF 1320 kb)
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.doi...........ae59feeb901f58d1c7a2a98539927457
- Full Text :
- https://doi.org/10.6084/m9.figshare.6616784