Microsatellite-Based Genetic Structure and Differentiation of Goldfish (Carassius auratus) with Sarcoma

Ten microsatellite loci were used for analyzing six populations of goldfish (Carassius auratus) with sarcoma. It showed that there was the highest genetic diversity among the white oranda with red cap (RC) population, and the lowest among the white tigerhead (WT) population. However, the outcross existed among every population. There was huge genetic differentiation between WT and the other four populations. The average observed heterozygosity (HO) among populations ranged from 0.3571 to 0.7381. And significant genetic difference (FCT = 0.1891, P = 0.0186) appeared among goldfish varieties which can be classified into three groups (RT, WT; RL, BL, YC; RC). The software Principal Coordinate Analysis (PCA) and STRUCTURE showed that significant genetic differences were revealed between RC population of goldfish and other five populations.