Real-Time Visualization of in Vitro Transcription of a Fluorescent RNA Aptamer: An Experiment for the Upper-Division Undergraduate or First-Year Graduate Laboratory

Authors :: Aaron E. Engelhart
Brent A. Heffron
Katarzyna P. Adamala
Jose Gomez-Garcia
Erin E. Carlson
B. Cash
Nathaniel J. Gaut
Lauren M. Aufdembrink
Joshua D. Shirley
Joseph M. Heili
Source :: Journal of Chemical Education. 95:1867-1871
Publication Year :: 2018
Publisher :: American Chemical Society (ACS), 2018.
Abstract: A teaching laboratory experiment is described where students prepare in vitro transcription reactions of a fluorescent RNA aptamer, named Broccoli, and observe the production of the aptamer in real...

Full Text Access

Tools