ACCLIMATIZATION OF DIANTHUS CARYOPHYLLUS LINN

In vitro shoots without roots from different media compositions were selected and transferred into test tubes supplemented with MS medium without hormone for root induction. After 4 weeks, full grown plantlets were transferred to sterile soil for acclimatization process. The plantlets were maintained in the culture room at 25±1°C with 16 hours light and 8 hours dark. Plantlets were then planted in pots and covered with polystyrene plastic bags. They grew well and after 4 weeks in the culture room, well grown and healthy plantlets were transferred to the greenhouse. Survival rate of the acclimatized plantlets was 70% and from observation it showed that most of the plantlets successfully survived after transferred to the greenhouse.