Binding of Ceftobiprole and Comparators to the Penicillin-Binding Proteins of Escherichia coli , Pseudomonas aeruginosa , Staphylococcus aureus , and Streptococcus pneumoniae

Ceftobiprole exhibited tight binding to PBP2a in methicillin-resistant Staphylococcus aureus , PBP2x in penicillin-resistant Streptococcus pneumoniae , and PBP3 and other essential penicillin-binding proteins in methicillin-susceptible S. aureus , Escherichia coli , and Pseudomonas aeruginosa . Ceftobiprole also bound well to PBP2 in the latter organisms, contributing to the broad-spectrum antibacterial activity against gram-negative and gram-positive bacteria.