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Gene-centromere mapping of 312 loci in pink salmon by half-tetrad analysis

Authors :
James E. Seeb
Chris Habicht
D J Reedy
Paul Spruell
Kathy L. Knudsen
K. R. Lindner
E Kretschmer
Fred W. Allendorf
Source :
Genome. 43:538-549
Publication Year :
2000
Publisher :
Canadian Science Publishing, 2000.

Abstract

We estimated recombination rates between 312 loci and their centromeres in gynogenetic diploid pink salmon (Oncorhynchus gorbuscha) that we produced by initiating development with irradiated sperm and blocking the maternal second meiotic division. Amplified fragment length polymorphisms (AFLPs) were significantly more centromeric than loci identified by three other techniques (allozymes, microsatellites, and PCR using primer sequences from interspersed nuclear elements). The near absence of AFLPs in distal regions could limit their utility in constructing linkage maps. A large proportion of loci had frequency of second division segregation (y) values approaching 1.0, indicating near complete crossover interference on many chromosome arms. As predicted from models of chromosomal evolution in salmonids based upon results with allozyme loci, all duplicated microsatellite loci that shared alleles (isoloci) had y values of nearly 1.0.Key words: meiosis, AFLP, microsatellites, isoloci, Oncorhynchus gorbuscha.

Details

ISSN :
14803321 and 08312796
Volume :
43
Database :
OpenAIRE
Journal :
Genome
Accession number :
edsair.doi...........b3ab9ddcbcf93ffb92745ba418716f5d
Full Text :
https://doi.org/10.1139/g00-016