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ECERIFERUM 10 Encoding an Enoyl-CoA Reductase Plays a Crucial Role in Osmotolerance and Cuticular Wax Loading in Arabidopsis

Authors :
Norika Fukuda
Yoshimi Oshima
Hirotaka Ariga
Takuma Kajino
Takashi Koyama
Yukio Yaguchi
Keisuke Tanaka
Izumi Yotsui
Yoichi Sakata
Teruaki Taji
Source :
Frontiers in Plant Science. 13
Publication Year :
2022
Publisher :
Frontiers Media SA, 2022.

Abstract

Acquired osmotolerance induced after salt stress is widespread acrossArabidopsis thaliana(Arabidopsis) accessions (e.g., Bu-5). However, it remains unclear how this osmotolerance is established. Here, we isolated a mutant showing an acquired osmotolerance-defective phenotype (aod2) from an ion-beam-mutagenized M2 population of Bu-5.aod2was impaired not only in acquired osmotolerance but also in osmo-shock, salt-shock, and long-term heat tolerances compared with Bu-5, and it displayed abnormal morphology, including small, wrinkled leaves, and zigzag-shaped stems. Genetic analyses ofaod2revealed that a 439-kbp region of chromosome 4 was translocated to chromosome 3 at the causal locus for the osmosensitive phenotype. The causal gene of theaod2phenotype was identical toECERIFERUM 10(CER10), which encodes an enoyl-coenzyme A reductase that is involved in the elongation reactions of very-long-chain fatty acids (VLCFAs) for subsequent derivatization into cuticular waxes, storage lipids, and sphingolipids. The major components of the cuticular wax were accumulated in response to osmotic stress in both Bu-5 WT andaod2. However, less fatty acids, primary alcohols, and aldehydes with chain length ≥ C30 were accumulated inaod2. In addition,aod2exhibited a dramatic reduction in the number of epicuticular wax crystals on its stems. Endoplasmic reticulum stress mediated bybZIP60was increased inaod2under osmotic stress. The onlycer10showed the most pronounced loss of epidermal cuticular wax and most osmosensitive phenotype among four Col-0-background cuticular wax-related mutants. Together, the present findings suggest thatCER10/AOD2plays a crucial role in Arabidopsis osmotolerance through VLCFA metabolism involved in cuticular wax formation and endocytic membrane trafficking.

Subjects

Subjects :
Plant Science

Details

ISSN :
1664462X
Volume :
13
Database :
OpenAIRE
Journal :
Frontiers in Plant Science
Accession number :
edsair.doi...........b4fad5b018b66b7e851f2c19d4c48a02
Full Text :
https://doi.org/10.3389/fpls.2022.898317