Back to Search
Start Over
Development of Lateral Flow Immunoassay for Antigen Detection in Human Angiostrongylus cantonensis Infection
- Source :
- The Korean Journal of Parasitology. 54:375-380
- Publication Year :
- 2016
- Publisher :
- Korean Society for Parasitology, 2016.
-
Abstract
- Angiostrongyliasis is difficult to be diagnosed for the reason that no ideal method can be used. Serologic tests require specific equipment and are not always available in poverty-stricken zone and are time-consuming. A lateral flow immunoassay (LFIA) may be useful for angiostrongyliasis control. We established a LFIA for the diagnosis of angiostrongyliasis based on 2 monoclonal antibodies (mAbs) against antigens of Angiostrongylus cantonensis adults. The sensitivity and specificity were 91.1% and 100% in LFIA, while those of commercial ELISA kit was 97.8% and 86.3%, respectively. Youden index was 0.91 in LFIA and 0.84 in commercial ELISA kit. LFIA showed detection limit of 1 ng/ml of A. cantonensis ES antigens. This LFIA was simple, rapid, highly sensitive and specific, which opened an alternative approach for the diagnosis of human angiostrongyliasis.
- Subjects :
- 0301 basic medicine
medicine.drug_class
030231 tropical medicine
030108 mycology & parasitology
Biology
Monoclonal antibody
Angiostrongylus cantonensis infection
medicine.disease
biology.organism_classification
Virology
Serology
Angiostrongylus cantonensis
03 medical and health sciences
Elisa kit
0302 clinical medicine
Infectious Diseases
Antigen
Immunology
medicine
Angiostrongyliasis
Parasitology
Lateral flow immunoassay
Subjects
Details
- ISSN :
- 17380006 and 00234001
- Volume :
- 54
- Database :
- OpenAIRE
- Journal :
- The Korean Journal of Parasitology
- Accession number :
- edsair.doi...........b569e9b9816c88963fe9fa3eadb7f183