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Spatiotemporal regulation of galectin-1-induced T-cell death in lamina propria from Crohn’s disease and ulcerative colitis patients
- Source :
- Apoptosis. 26:323-337
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Inflammatory bowel disease (IBD), including Crohn’s disease (CD) and ulcerative colitis (UC), is characterized by chronic, relapsing intestinal inflammation. Galectin-1 (Gal-1) is an endogenous lectin with key pro-resolving roles, including induction of T-cell apoptosis and secretion of immunosuppressive cytokines. Despite considerable progress, the relevance of Gal-1-induced T-cell death in inflamed tissue from human IBD patients has not been ascertained. Intestinal biopsies and surgical specimens from control patients (n = 52) and patients with active or inactive IBD (n = 97) were studied. Gal-1 expression was studied by RT-qPCR, immunoblotting, ELISA and immunohistochemistry. Gal-1-specific ligands and Gal-1-induced apoptosis of lamina propria (LP) T-cells were determined by TUNEL and flow cytometry. We found a transient expression of asialo core 1-O-glycans in LP T-cells from inflamed areas (p
- Subjects :
- 0301 basic medicine
Peanut agglutinin
Cancer Research
Clinical Biochemistry
Pharmaceutical Science
Inflammation
Inflammatory bowel disease
03 medical and health sciences
0302 clinical medicine
medicine
Pharmacology
Lamina propria
Crohn's disease
biology
business.industry
Biochemistry (medical)
Cell Biology
medicine.disease
Ulcerative colitis
030104 developmental biology
medicine.anatomical_structure
030220 oncology & carcinogenesis
Immunology
biology.protein
Immunohistochemistry
medicine.symptom
business
CD8
Subjects
Details
- ISSN :
- 1573675X and 13608185
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- Apoptosis
- Accession number :
- edsair.doi...........b62285a764a299dda33450dfc0cdf8ac