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Isobaric tags for relative and absolute quantification-based proteomic analysis that reveals the roles of progesterone receptor, inflammation, and fibrosis for slow-transit constipation
- Source :
- Journal of Gastroenterology and Hepatology. 33:385-392
- Publication Year :
- 2018
- Publisher :
- Wiley, 2018.
-
Abstract
- Background and Aim Progesterone receptor, inflammation, neurotransmitter expression, and fibrosis are involved in slow-transit constipation. The aim of the present study was to examine whether patients with slow-transit constipation have an overexpression of progesterone receptor and serotonin which may impair the fibrosis of muscularis propria in colorectal wall. Methods High resolution colon manometry was used to record the colorectal peristaltic contractions of the proximal ascending and sigmoid colon in patients. Protein samples prepared from frozen sigmoid colon tissue and the proximal margin of the ascending colon of four female patients were compared using iTRAQ labeling technique coupled to 2D LC–MS/MS analysis. Immunohistochemical staining of progesterone receptor, serotonin, and fibronectin was performed in paraffin-embedded sigmoid colon tissues and the proximal margin of the ascending colon or ileum from 43 patients with slow-transit constipation. Results Among these differentially regulated proteins based on iTRAQ and LC–MS/MS analysis, 56 proteins involved in the response to progesterone, inflammation, matrix remodeling, fibrosis, and muscle metabolism. Immunohistochemical staining confirmed that there was significantly higher expression of progesterone receptor (t = 19.19, P = 0.000) and serotonin (t = 13.52, P = 0.004) in sigmoid colon than in the proximal margin of the ascending colon and ileum. Progesterone receptor and fibronectin expression in the outer layer of muscularis propria was higher than in the middle layer. Conclusions These results demonstrate that progesterone receptor, along with inflammation and fibrosis may take part in slow-transit constipation development.
- Subjects :
- Pathology
medicine.medical_specialty
Constipation
Hepatology
business.industry
Gastroenterology
Sigmoid colon
Inflammation
Ileum
medicine.disease
digestive system diseases
03 medical and health sciences
0302 clinical medicine
medicine.anatomical_structure
Fibrosis
030220 oncology & carcinogenesis
Progesterone receptor
medicine
Immunohistochemistry
Ascending colon
030211 gastroenterology & hepatology
medicine.symptom
business
Subjects
Details
- ISSN :
- 08159319
- Volume :
- 33
- Database :
- OpenAIRE
- Journal :
- Journal of Gastroenterology and Hepatology
- Accession number :
- edsair.doi...........b6727b4de3aebe43b8525c67d2301eb5