Seeding nucleofected hPSCs in 96-well plates using limited dilution v1

This protocol describes a standard procedure for seeding nucleofected human pluripotent stem cells in 96-well plates using limited dilution. This protocol follows nucleofection of hPSCs as described in detail in the collection "Nucleofection (Amaxa) andelectroporation(Biorad) of hPSCs;" dx.doi.org/10.17504/protocols.io.b4qnqvve General notes 1. Throughout this protocol, the term hPSC is used to collectively refer to both hiPSCs and hESCs. All described procedures have been tested and work equally well for hiPSCs and hESCs.