Back to Search
Start Over
Effect of arsenic trioxide or ATRA on primary APL cell or HL-60 cells and their value analysis in hyperleukocytosis
- Source :
- Chinese Journal of Cancer Research. 13:119-123
- Publication Year :
- 2001
- Publisher :
- Chinese Journal of Cancer Research, 2001.
-
Abstract
- Objective: To detect the effect of arsenic trioxide or ATRA on APL cells or HL-60 cells and to investigate the mechanism of the hyperleukocytosis and detect the cross resistance between ATRA and arsenic trioxide. Methods: The number of promyelocytes or more matured granulocytes were counted by regular method, MTT test was used to measure the proliferation of HL-60 cells or APL cells, flow cytometry analysis to measure the apoptosis, NBT method to detect the differentiation of HL-60 cells or APL cells. Results: The proliferation of primary APL cells or HL-60 cells could be inhibited in vitro by either arsenic trioxide or ATRA, which could induce obvious apoptosis or obvious differentiation of primary APL cells or HL-60 cells. Inhibition of proliferation or apoptosis of ATRA resistant HL-60 cells were achieved by exposure to arsenic trioxide in vitro. On the other hand, the results of in vivo treatment showed that arsenic trioxide also induce of hyperleukocytosis. Conclusion: The results indicated that the hyperleukocytosis induced by ATRA is not contributed to the mechanism of more differentiation than apoptosis, there was not cross resistance between ATRA and arsenic trioxide.
Details
- ISSN :
- 19930631 and 10009604
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Chinese Journal of Cancer Research
- Accession number :
- edsair.doi...........b9f0d64d7ddb27de9c7180e5c15a6d2e