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Nanomagnetic Particle Anchored to Monodispers Porous Microspheres for Pectinase Immobilization

Authors :
Xiao Liang Zhang
Shu Xian Bi
Source :
Advanced Materials Research. 1088:38-42
Publication Year :
2015
Publisher :
Trans Tech Publications, Ltd., 2015.

Abstract

The idea of preparing the surface of chitosan layer pre-absorbed on the monodispers porous microsphere via layer by layer approach receives special relevance in enzyme technology. Moreover, the colloidal stability is not impeded by the adsorbed proteins despite the fact that up to 390.2 mg of enzyme adsorbed per gram of the carrier particles. Maximum relative activity was observed at 50 °C and pH 3.5. The activity half lives for native and bound states of enzyme were found as 14.27 d and 31.05 d, respectively. These particles, premodified with the layer of magnetic nanoparticles to impart the magnetic property, were repeatedly used as catalysts following their rapid and easy separation.

Details

ISSN :
16628985
Volume :
1088
Database :
OpenAIRE
Journal :
Advanced Materials Research
Accession number :
edsair.doi...........ba0c069219335d89ff1c9c18fec42e89
Full Text :
https://doi.org/10.4028/www.scientific.net/amr.1088.38