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Preparation of peptone from chicken bone residue by using natural pancreas as catalyst
- Source :
- Journal of Chemical Technology & Biotechnology. 91:2852-2861
- Publication Year :
- 2016
- Publisher :
- Wiley, 2016.
-
Abstract
- BACKGROUND The cost of peptone is often a major contributor to costs in the fermentation industry. A novel method for preparation of peptone from chicken bone residue by using natural pancreas as catalyst was developed in this study, aiming to provide a way to convert waste into valuable peptone. RESULTS The molecular weight of chicken bone extract (CBE) hydrolyzed with pancreas or its combination with trypsin ranges between 100 and 1000 Da after 4 h of hydrolysis. CBE hydrolyzed with pancreas (5%, w/w) for 4 h shows higher degree of hydrolysis (DH) (23.60%) than that of 0.5% trypsin, and higher polypeptides and free amino acids content than that of 0.5% trypsin and 1% pancreas. Thus, it was selected for yeast, bacteria and fungi incubation, with commercial casein peptone as a control. Saccharomyces cerevisiae shows a similar growth curve in both media during 36 h incubation. Although Bacillus subtilis shows a longer lag phase (14 h) in chicken bone hydrolysate (CBH) than in the control (6 h), significantly higher OD600 in CBH was obtained after 24 h incubation (P
- Subjects :
- 0106 biological sciences
0301 basic medicine
General Chemical Engineering
030106 microbiology
Biology
01 natural sciences
Hydrolysate
Inorganic Chemistry
03 medical and health sciences
Hydrolysis
Aspergillus oryzae
010608 biotechnology
Casein
medicine
Food science
Waste Management and Disposal
Incubation
Renewable Energy, Sustainability and the Environment
Organic Chemistry
Trypsin
biology.organism_classification
Pollution
Yeast
Fuel Technology
Biochemistry
Fermentation
Biotechnology
medicine.drug
Subjects
Details
- ISSN :
- 02682575
- Volume :
- 91
- Database :
- OpenAIRE
- Journal :
- Journal of Chemical Technology & Biotechnology
- Accession number :
- edsair.doi...........bad18777028f86f99addd75fbea7f33b