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Pyruvate metabolism in rice coleoptiles under anaerobiosis

Authors :
Hisashi Kato-Noguchi
Source :
Plant Growth Regulation. 50:41-46
Publication Year :
2006
Publisher :
Springer Science and Business Media LLC, 2006.

Abstract

Relative importance of ethanolic, lactate and alanine fermentation pathways was estimated in coleoptiles of rice seedlings (Oryza sativa L.) subjected to anoxic stress. The in vitro activities of alcohol dehydrogenase (ADH, EC 1.1.1.1), pyruvate decarboxylase (PDC, EC 4.1.1.1) and alanine aminotransferase (AlaAT, EC 2.6.1.2) in the coleoptiles increased in anoxia, whereas no significant increase was measured in lactate dehydrogenase (LDH, EC 1.1.1.27) activity. At 48 h, the ADH, PDC and AlaAT activities in anoxic coleoptiles were 62-, 15- and 7.6-fold greater, respectively, than those in the presence of oxygen. Ethanol and alanine in the coleoptiles accumulated rapidly under anoxia, increasing by 48 h, 57- and 5.6-fold compared with those in the presence of oxygen, respectively. However, lactate concentration did not increase and no initial burst of lactate production was detected. The relative ratio of carbon flux from pyruvate to ethanol, lactate and alanine in anoxic coleoptiles was estimated to be 92, 1 and 7% of the total carbon flux, respectively. These results suggest that the potential carbon flux from pyruvate to ethanol may be much greater than the potential flux from pyruvate to lactate and alanine in rice coleoptiles during anoxia.

Details

ISSN :
15735087 and 01676903
Volume :
50
Database :
OpenAIRE
Journal :
Plant Growth Regulation
Accession number :
edsair.doi...........bb29ddf03ba8beb919b6f678b50f19c0
Full Text :
https://doi.org/10.1007/s10725-006-9124-4